Recombinant Schistosoma japonicum 23 kDa integral membrane protein, partial

Code CSB-YP326733SXP1
MSDS
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Source Yeast
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Code CSB-EP326733SXP1
MSDS
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Source E.coli
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Code CSB-EP326733SXP1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326733SXP1
MSDS
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Source Baculovirus
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Code CSB-MP326733SXP1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
23 kDa integral membrane protein; Sj23
Species
Schistosoma japonicum (Blood fluke)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I'm intersted in CSB-EP326733SXP1 and my concerns and requests:
I had revisions with my materials and methods. I would like to know if you can quote me for the 8.5 kDa part of extracellular domain II of Sj23 at 1 mg, E. coli expression host. PHilippine strain is preferred, but not required.
Also, may I know what other proteins from Schistosoma japonicum can you clone? I prefer tegument proteins or excretory/secretory proteins from the larval stage of the parasite (cercaria). How much is it at 1 mg?”

A:
Thanks for your inquiry.
Recombinant Schistosoma japonicum 23 kDa integral membrane protein,partial
CSB-EP326733SXP1 >> E.coli
Expression Region: 109-183aa; Partial.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

KDKIDDEINTLMTGALENPNEEITATMDKIQTSFHCCGVKGPDDYKGNVPASCKEGQEVYVQGCLSVFSAFLKRN


As to the 8.5 kDa part of extracellular domain II of Sj23, do you refer to the region of 109-183aa?
In addition, according to your description that I prefer tegument proteins or excretory/secretory proteins from the larval stage of the parasite (cercaria),
We think you may need the native protein, if so, sorry, we can't provide this native protein.

Target Background

Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Tetraspanin (TM4SF) family
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