Recombinant Schizosaccharomyces pombe Actin-related protein 3 (act2)

Code CSB-YP001249SXV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP001249SXV
MSDS
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Source E.coli
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Code CSB-EP001249SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001249SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP001249SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
act2
Uniprot No.
Alternative Names
act2; SPAC630.03; Actin-related protein 3; Actin-like protein 3
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-427
Target Protein Sequence
MASFNVPIIM DNGTGYSKLG YAGNDAPSYV FPTVIATRSA GASSGPAVSS KPSYMASKGS GHLSSKRATE DLDFFIGNDA LKKASAGYSL DYPIRHGQIE NWDHMERFWQ QSLFKYLRCE PEDHYFLLTE PPLNPPENRE NTAEIMFESF NCAGLYIAVQ AVLALAASWT SSKVTDRSLT GTVVDSGDGV THIIPVAEGY VIGSSIKTMP LAGRDVTYFV QSLLRDRNEP DSSLKTAERI KEECCYVCPD IVKEFSRFDR EPDRYLKYAS ESITGHSTTI DVGFERFLAP EIFFNPEIAS SDFLTPLPEL VDNVVQSSPI DVRKGLYKNI VLSGGSTLFK NFGNRLQRDL KRIVDERIHR SEMLSGAKSG GVDVNVISHK RQRNAVWFGG SLLAQTPEFG SYCHTKADYE EYGASIARRY QIFGNSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. May be involved in cytokinesis.
Gene References into Functions
  1. WASp-VCA binding to Arp2/3 protein complex causes a conformational change that favors binding to the side of an actin filament, which allows further changes required to nucleate a daughter filament. PMID: 30150414
  2. ARP3 role in the actin filament nucleation.ATP binding destabilizes this interaction via an allosteric link between the Arp3 nucleotide cleft and the hydrophobic groove, thereby promoting the short-pitch conformation. PMID: 27417392
  3. Arp2/3 complex participates in regulating formin F-actin networks. PMID: 24560576
  4. Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex PMID: 23148219
  5. Truncation of the N terminus of ARPC5, the smallest subunit in the complex, increased the yield of Arp2Delta Arp2/3 complex during purification but did not compromise nucleation activity of the full Arp2/3 complex. PMID: 18640983

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Subcellular Location
Cytoplasm, cytoskeleton, actin patch.
Protein Families
Actin family, ARP3 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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