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Recombinant Schizosaccharomyces pombe Cell cycle serine/threonine-protein kinase hsk1 (hsk1), partial

Recombinant Schizosaccharomyces pombe Cell cycle serine/threonine-protein kinase hsk1 (hsk1), partial

Code	CSB-YP343866SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP343866SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP343866SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP343866SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP343866SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

hsk1

Uniprot No.

P50582

Alternative Names

hsk1; SPBC776.12cCell cycle serine/threonine-protein kinase hsk1; EC 2.7.11.1; Cdc7-related kinase; Minichromosome maintenance protein kinase

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for G1/S transition. Plays a role in DNA replication checkpoint signaling through regulating rad3 and cds1. Involved in the maintenance of mitotic chromosome structures during S phase through regulating the function of rad21. Required for initiation of mitotic DNA replication through phosphorylating mcm2/cdc19. Required for genome integrity.

Gene References into Functions

Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation PMID: 22024164
Hsk1 kinase activity is required for induction and maintenance of Mrc1 hyperphosphorylation, which is induced by replication fork block and mediated by Rad3. PMID: 21099360
Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1. PMID: 20176980
Swi1, Swi3, and Hsk1 act in a novel S-phase checkpoint pathway PMID: 15767681
Hsk1 kinase has roles in the initiation of meiotic recombination and meiosis PMID: 16698922
These results support a model in which the accessibility of replication origins to Hsk1-Dfp1 regulates origin efficiency and provides a potential mechanistic link between chromatin structure and replication timing. PMID: 18799612
These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast. PMID: 19422421