Recombinant Schizosaccharomyces pombe Cell division cycle protein cdt1 (cdt1)

Code CSB-YP331160SXV
MSDS
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Source Yeast
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Code CSB-EP331160SXV
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Source E.coli
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Code CSB-EP331160SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331160SXV
MSDS
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Source Baculovirus
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Code CSB-MP331160SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cdt1
Uniprot No.
Alternative Names
cdt1; SPBC428.18Cell division cycle protein cdt1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-444
Target Protein Sequence
MKCRALYSHD NNILIFGFCV QIMSAGSQTK LNFSVRKTRS SSKRSNAAII EPPKNPEDSQ IIPAVKRLKE NLDTESLEQN EVLPPVKNES VLFLEKVFNA VDICVKFHLS INTKPTFVLL ENKVSGLTKI SLKITHLAQI LTVWPESFAI TPCFTIHQGK RVATYELSYP RNANLPEAFS RSIEFKRRLE KWLLEHCSET EIPAQQLQAL PSLSKNTVNE SSLVRKLNLE KSTSRELRIP TQTLEPKFTT NTAKYANELV SCSMLDSSST LSKSVNSKIN LKSHQSSSSV QNSSRKLTSS QLTLRQSSLF DRVRKKQKAM EAKKAEEFKN NLVVHTLAKE KVSFVRIIDL IFVQLSTWPT KRSFSMSEIV TSMQMSISSS LSPDQCAKAI ELLSKALPAW CTINLLGNIQ VVTFSRIVNG KPYLRSQLIE ELQTKASITI LSNS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM helicase complex to the replication origins.
Gene References into Functions
  1. we show that in Schizosaccharomyces pombe, Cdt1 is proteolysed in M and G1 phases in response to DNA damage and that this mechanism seems to be conserved from yeast to Metazoa. PMID: 17039252
Subcellular Location
Nucleus. Note=Present in the nucleus after mitosis when DNA licensing takes place.
Protein Families
Cdt1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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