Recombinant Schizosaccharomyces pombe DNA repair protein rad60 (rad60)

Code CSB-YP891502SXV
MSDS
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Source Yeast
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Code CSB-EP891502SXV
MSDS
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Source E.coli
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Code CSB-EP891502SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891502SXV
MSDS
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Source Baculovirus
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Code CSB-MP891502SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rad60
Uniprot No.
Alternative Names
rad60; SPBC1921.02; DNA repair protein rad60
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-406
Target Protein Sequence
MDNLDEDDLA FFSKPIKKPP LNYAKQLIAS SSDSEEESEL DTNKQALEHI NAQKNITHNE NKSAEPLSRQ STILDADEGN QDVSDTTPNA CLNEGRHSPK SAISCVTQPV SPVYNTRAAA NLRNNSINSE AALSTTSSLL DDDFARRLEE IDRQVQEFEK SSSDMDVQIH THKREIEEDD DNTSADVPLL KHSKSDHSTL YHSKSEFSTN EPVISVVLQL AVIGQRIPNS NISLPRDWEA PLFFKVKSNQ QFRRVRIAYS ERKKVDNVVL VFQNQRLWDY GTPKGAGMLK VDTRLVVHAY CHSDFISLKR IKELEVEKLS SVTEDSTAQT CKLITLLLRS SKSEDLRLSI PVDFTVKDLI KRYCTEVKIS FHERIRLEFE GEWLDPNDQV QSTELEDEDQ VSVVLD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.
Gene References into Functions
  1. These results suggest that Rhp51 and Rad60 are required for the restoration of a stalled or collapsed replication fork after release from the arrest of DNA replication [Rhb51][Rad60] PMID: 16354704
  2. role in protecting genome stability against spontaneous Top1-mediated DNA damage PMID: 21408210
  3. SUMO-like domains 1 of Rad60 is essential for viability, while SUMO-like domains 2 is not. PMID: 20885950
  4. results provide a mechanistic basis for the near-synonymous roles of Rad60 and SUMO in survival of genotoxic stress and suggest unprecedented DNA-damage-response functions for SLDs in regulating sumoylation PMID: 19363481
Subcellular Location
Nucleus.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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