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Recombinant Schizosaccharomyces pombe DNA replication licensing factor mcm10 (mcm10), partial

Recombinant Schizosaccharomyces pombe DNA replication licensing factor mcm10 (mcm10), partial

Code	CSB-YP520370SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP520370SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP520370SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP520370SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP520370SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

mcm10

Uniprot No.

O42709

Alternative Names

mcm10; cdc23; SPBC1347.10DNA replication licensing factor mcm10; Cdc23 protein; Minichromosome maintenance protein 10

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for DNA synthesis. Required for entry into or completion of S phase. Involved in DNA replication and seems to participate in the activation of the pre-replication complex (pre-RC) and in transcription elongation. May play a role as key coordinator in assembling the replication fork. Proposed to function at replication origins following the binding of the mcm2-7 complex prior to the recruitment of sna41. Probably is required to stimulate phosphorylation of the mcm2-7 complex by the dfp1-hsk1 kinase complex. May recruit the DNA polymerase alpha:primase complex to replication origins and is required to maintain it on chromatin independently of sna41. May have primase activity. Binds to single-stranded DNA.

Gene References into Functions

Data collection at lambda = 2.69 A (4.6 keV) allowed the crystal structure determination by sulfur SAD phasing of Cdc23(Nterm), a subunit of the multimeric anaphase-promoting complex (APC/C). At this energy, Cdc23(Nterm) has an expected Bijvoet ratio<|Fanom|>/of 2.2%, with 282 residues, including six cysteines and five methionine residues, and two molecules in the asymmetric unit (65.4 kDa; 12 Cys and ten Met residu... PMID: 26960127
The interaction of Mcm10p/Cdc23p with the DNA polymerase alpha-primase complex, its binding to single-stranded DNA, and its activation of the polymerase complex together contribute to its role in the elongation phase of DNA replication. [mcm10] PMID: 14766746
The C terminus of Mcm10p synthesized RNA, but less efficiently than the full-length protein at low rNTP levels; primase activity of Mcm10p may be essential for cell viability PMID: 16720577

Subcellular Location

Nucleus. Note=Associates with chromatin.

Protein Families

MCM10 family

Database Links

KEGG: spo:SPBC1347.10

STRING: 4896.SPBC1347.10.1

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