Recombinant Schizosaccharomyces pombe E3 SUMO-protein ligase pli1 (pli1), partial

Code CSB-YP528446SXV
MSDS
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Source Yeast
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Code CSB-EP528446SXV
MSDS
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Source E.coli
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Code CSB-EP528446SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528446SXV
MSDS
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Source Baculovirus
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Code CSB-MP528446SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pli1
Uniprot No.
Alternative Names
pli1; SPAC1687.05; E3 SUMO-protein ligase pli1; EC 2.3.2.-; E3 SUMO-protein transferase plil
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as an E3 ligase mediating SUMO/Smt3 attachment to other proteins. Involved in the maintenance of the centromere and in telomere length. Regulates recombination, via extension sumoylation, particularly within the heterochromatin repeats.
Gene References into Functions
  1. study identifies the existence of an additional DNA interstrand cross-link (ICL) resolution pathway dependent on the SUMO E3 ligase Pli1 PMID: 24192486
Subcellular Location
Nucleus.
Protein Families
PIAS family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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