Recombinant Schizosaccharomyces pombe GTP-binding protein ypt7 (ypt7)

Code CSB-YP529717SXV
MSDS
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Source Yeast
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Code CSB-EP529717SXV
MSDS
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Source E.coli
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Code CSB-EP529717SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529717SXV
MSDS
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Source Baculovirus
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Code CSB-MP529717SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ypt7
Uniprot No.
Alternative Names
ypt7; SPBC405.04cGTP-binding protein ypt7
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-205
Target Protein Sequence
MAGKKKHLLK VIILGESGVG KTSIMNQYVN RKFSKDYKAT IGADFLTKEV LVDDKVVTLQ LWDTAGQERF QSLGVAFYRG ADCCVLVYDV NNSKSFETLD SWRDEFLIQA SPSNPETFPF ILLGNKVDVE EQKRMVSKSK ALAFCQARGE IPYFETSAKE AINVQEAFET VAKLALENMD SDDIAADFTD PIHLDMESQK TSCYC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion. Ypt7 is necessary for trafficking from the endosome to the vacuole and for homotypic vacuole fusion. Plays an important role in sporulation.
Gene References into Functions
  1. Here, we report that Ypt7p plays important roles in sporulation. PMID: 16357443
  2. Data show that the two S. pombe Rab7 homologs, Ypt7 and Ypt71, act antagonistically in regulating vacuolar morphology. PMID: 19453973
Subcellular Location
Vacuole membrane.
Protein Families
Small GTPase superfamily, Rab family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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