Recombinant Schizosaccharomyces pombe Histone acetyltransferase gcn5 (gcn5)

Code CSB-YP891658SXV
MSDS
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Source Yeast
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Code CSB-EP891658SXV
MSDS
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Source E.coli
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Code CSB-EP891658SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891658SXV
MSDS
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Source Baculovirus
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Code CSB-MP891658SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gcn5
Uniprot No.
Alternative Names
gcn5; SPAC1952.05Histone acetyltransferase gcn5; EC 2.3.1.48
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-454
Target Protein Sequence
MSNSLNDQTR PPDSSVVDST SSNFPNASND SVHDKPVKQD EQSNPNVREA IANSDGQESL AVKENKDTES SSSHFVPNGV SNSKKRKLVA TDLDVDFDIS SVRVTEKPSV LEEKSGVIQF RVVSNDDTAD SMIMLTGLKN IFMKQLPKMP KEYITRLIYD RNHLSMTIVK DNLHVVGGIT YRPFEQRGFA EIVFCAIASN EQVRGYGSHL MNHLKDYVRG TTTIQHFLTY ADNYAIGYFK KQGFTKEITL DKSIWVGYIK DYEGGTLMQC TMIPKIKYLE ANLILAIQKA AVVSKINRIT RSNVVYPGLD VFKDGPAHIE PSQVPGLMEV GWCKEMEELS KKPRPKPFFA VLEMLFTEMQ NHPSSWPFMQ PVSKEDVPDY YEVIEHPMDL STMEFRLRNN QYESVEEFIR DAKYIFDNCR SYNDSNTTYY KNADRLEKFF QKKLRETEYS HLAD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acetylates histone H2B to form H2BK11ac and H2BK16ac, histone H3 to form H3K14ac, and with a lower preference histone H4 to form H4K8ac and H4K16ac. Acetylation of histones gives a specific tag for epigenetic transcription activation.
Gene References into Functions
  1. Gcn5 mediates eviction of nucleosomes positioned downstream of promoters, allowing efficient Pol II progression along the genes. PMID: 21515633
  2. The histone acetyltransferase Gcn5 is required for most histone H3 acetylation around the meiotic recombination hotspot M26. Deletion of gcn5 causes a significant delay in chromatin remodeling but only partial reduction of M26 meiotic recomb. freq. [gcn5] PMID: 14988732
Subcellular Location
Nucleus.
Protein Families
Acetyltransferase family, GCN5 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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