Recombinant Schizosaccharomyces pombe Kinetochore protein mis17 (mis17)

Code CSB-YP527244SXV
MSDS
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Source Yeast
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Code CSB-EP527244SXV
MSDS
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Source E.coli
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Code CSB-EP527244SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527244SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP527244SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mis17
Uniprot No.
Alternative Names
mis17; SPBC21.01; SPBC776.19Inner kinetochore subunit mis17; CENP-U homolog; Constitutive centromere-associated network protein mis17; Sim4 complex subunit mis17
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-441
Target Protein Sequence
MENNSHNVDE RRAARMRGAE RYKIQNVEFS LDQNLLANLE SSTSSSTRSS PASNQQNLEI VSQSEAYHKI SDLDDSTSIF SISDQENILE RRISEEVVRE NSVSSIENVS SSAVARETQS SANILVKDNH FFSHKQKKIQ RESIPFHKRD NIETSDAYSS SILENSPPNK VQRLSSLDSS QDSFQEEHPG NVTGTTFSSQ APEERIASPI STSSPESLTN QSSSLQSSLQ TSSMAPRNLL DQSTEKDIVV GASDELVRIE LTKLSKEAGG SKTLNELDAV QQFFQEFIKE NEPLEPYVVK VKNAFVEQVS IRLLELIDLL DANRILSTAC KKAANEKLAV QRDLSKLRED RLSVQRKKIQ LRNEYIKLSH QQNFLDDIDD FFSQCETVKN ELENVTTTPN STEAFSEINE YASALSKNYG LESQLVELQS LLTQFYQKFL S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.
Subcellular Location
Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore.
Protein Families
CENP-U/AME1 family
Database Links
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