Recombinant Schizosaccharomyces pombe Meiotic coiled-coil protein 6 (mcp6)

Code CSB-YP607392SXV
MSDS
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Source Yeast
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Code CSB-EP607392SXV
MSDS
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Source E.coli
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Code CSB-EP607392SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP607392SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP607392SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mcp6
Uniprot No.
Alternative Names
mcp6; hrs1; mug3; SPBC582.06cMeiotic coiled-coil protein 6; Horsetail movement protein hrs1; Meiotically up-regulated gene 3 protein
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-327
Target Protein Sequence
MEYQEEASLA SAEDSTFIAS SPVQSVSEMK SIKQKSFQYF DDYEKNVSDK TIQFQKLQMT AKEIIDAFER DSTQRTLQIE SLESKIGEQE RDLNNEKLAS ETLREKTQLL EKENGALKVE NGYLYEKSRK LEEEMAHLKK KCNVYKSKFE ESSLRCKSLY SSNTKLKDSM ETMKRRMETE TKEMNKIKPK NDSESDRFKR NSQSLSQQSP LLDVHSPDNS NHRTMLNINN SSPIKPKKIF KPNEVKNRIS RLQKTFADLE NQHHSFQQIC QTLRKRLEND SSTTKQRLSK LEEIIRNRAP PSYSFSLNCS HTYQPVSCVE PVNHDLS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has a role in meiotic nuclear oscillation and recombination. Required to remodel astral microtubules into the 'horsetail' astral array maintaining the 'horsetail' nuclear movement. Promotes homologous paring of chromosomes during this movement.
Gene References into Functions
  1. Mcp6 (meiotic coiled-coil protein 6), which is a meiosis-specific, coiled-coil protein that localizes to the spindle-pole body (SPB), controls horsetail movement by regulating the astral microtubule organization during meiosis. [mcp6] PMID: 15654021
  2. Hrs1p/Mcp6p on the meiotic spindle pole body organizes astral microtubule arrays for oscillatory nuclear movement. PMID: 16111942
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Note=Localizes to the spindle pole body until the onset of meiosis I.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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