Recombinant Schizosaccharomyces pombe Mitotic spindle checkpoint component mad2 (mad2)

Code CSB-YP519398SXV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP519398SXV
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP519398SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519398SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP519398SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mad2
Uniprot No.
Alternative Names
mad2; SPBC20F10.06Mitotic spindle checkpoint component mad2
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-203
Target Protein Sequence
MSSVPIRTNF SLKGSSKLVS EFFEYAVNSI LFQRGIYPAE DFKVVRKYGL NMLVSVDEEV KTYIRKIVSQ LHKWMFAKKI QKLILVITSK CSGEDLERWQ FNVEMVDTAD QFQNIGNKED ELRVQKEIQA LIRQITATVT FLPQLEEQCT FNVLVYADKD SEVPTDWVDS DPRILRDAEQ VQLRSFSTSM HKIDCQVAYR VNP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. It interacts with the anaphase promoting complex/cyclosome (APC/C) thereby inhibiting APC/C-dependent proteolysis, a step required for exit from mitosis.
Gene References into Functions
  1. Results provide evidence that the truncation of two beta strands (beta7 and beta8) of Mad2 destabilize the safety belt and affect the Cdc20-Mad2 interaction leading to defects in the spindle checkpoint activation. PMID: 27664031
  2. Mad2p and Slp1p act downstream of Rad3p in the DNA replication checkpoint and Mad2p is required for the DNA replication checkpoint when Cds1p is compromised PMID: 15347659
  3. Mis6-complex, in collaboration with the Nuf2-complex, monitors the spindle-kinetochore attachment state and acts as a platform for Mad2 to accumulate at unattached kinetochores PMID: 15930132
  4. findings indicate that altered Mad2p anaphase spindle localizations can reflect changes in spindle function during mitotic exit that could contribute to fidelity in anaphase events PMID: 16855399
  5. When overexpressed ectopically, mutant Mad2 proteins sequestered wild-type Mad2, preventing its function in mitotic checkpoint arrest, but not in minichromosome stability. PMID: 17277378
  6. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p. PMID: 19666000

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Subcellular Location
Nucleus.
Protein Families
MAD2 family
Database Links
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