Recombinant Schizosaccharomyces pombe Mitotic spindle checkpoint component mad3 (mad3)

Code CSB-YP524569SXV
MSDS
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Source Yeast
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Code CSB-EP524569SXV
MSDS
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Source E.coli
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Code CSB-EP524569SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP524569SXV
MSDS
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Source Baculovirus
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Code CSB-MP524569SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mad3
Uniprot No.
Alternative Names
mad3; SPCC1795.01c; SPCC895.02Mitotic spindle checkpoint component mad3
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-310
Target Protein Sequence
MEPLDAGKNW VHMDVIEQSK ENIEPRKAGH SASALAKSSS RNHTEKEVAG LQKERMGHER KIETSESLDD PLQVWIDYIK WTLDNFPQGE TKTSGLVTLL ERCTREFVRN PLYKDDVRYL RIWMQYVNYI DEPVELFSFL AHHHIGQESS IFYEEYANYF ESRGLFQKAD EVYQKGKRMK AKPFLRFQQK YQQFTHRWLE FAPQSFSSNT NSVNPLQTTF ESTNIQEISQ SRTKISKPKF KFSVYSDADG SGKDGQPGTW QTLGTVDQRR KENNISATSW VGEKLPLKSP RKLDPLGKFQ VHCDEEVSKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a role in transducing the anaphase inhibitory signal to the anaphase promoting complex (APC). Forms part of the mad2 feedback control.
Gene References into Functions
  1. A kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and levels of anaphase promoting complex-associated Mad2 and Mad3 are dramatically reduced in this mutant. PMID: 22281223
  2. the Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset PMID: 18556659
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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