Recombinant Schizosaccharomyces pombe Negative regulator of ofd1 (nro1)

Code CSB-YP892388SXV
MSDS
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Source Yeast
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Code CSB-EP892388SXV
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Source E.coli
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Code CSB-EP892388SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892388SXV
MSDS
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Source Baculovirus
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Code CSB-MP892388SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nro1
Uniprot No.
Alternative Names
nro1; SPCC4B3.07; Negative regulator of ofd1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-393
Target Protein Sequence
MIGRRPQGLR AAASLKKQQQ LEKQKQEASY ELSGNSSPSK ENGSENVDNG EMEDETMLVY TEEDNISQLW GLYEMSREKL ENDDIDASVS LVFGTIHEAD RILRNTEDIS TLPKDFHAAY SSALLAVSEL FEIAQKRLKE TNTEESYIDA AIERAQLGLD APGNESRLFL ALARAYLEKV RVLVWRHDNE ESLANIPVTQ LVNPYIEKAI QYLRPLAQDS TEYFDALTPD SLRPLYILSS YLFQFGDQFS EAFLLDVCSI ITALWLKSVV DPNTPAYYKL IAQEAVLNNY TTFAEYYMDL LDNSESNVDD LINKASSWLN NSVDTWNVIY TLDKSPERLL KLADIKMDLA QIVQDEASQD NYLKEACNAI KEAQGSGVEL SPDYVEFVEA YSA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for correct translation termination. Positive regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression. Acts also as a direct inhibitor of ofd1. Functions probably by inhibiting the ability of the ofd1 to accelerate Sre1N degradation in absence of oxygen.
Gene References into Functions
  1. Nro1 unexpectedly adopts a Tetratrico Peptide Repeat (TPR) fold, a motif often responsible for protein or peptide binding. PMID: 21610214
  2. Studies indicate that the Nro1 NTD binds Ofd1CTDD and stabilizes Sre1N. PMID: 21481773
  3. we identify Nro1 (SPCC4B3.07) as a positive regulator of Sre1N stability and a direct inhibitor of Ofd1. PMID: 19158663
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
ETT1 family
Database Links
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