Recombinant Schizosaccharomyces pombe Probable E3 ubiquitin-protein ligase dma1 (dma1)

Code CSB-YP608776SXV
MSDS
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Source Yeast
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Code CSB-EP608776SXV
MSDS
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Source E.coli
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Code CSB-EP608776SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP608776SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP608776SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dma1
Uniprot No.
Alternative Names
dma1; SPAC17G8.10cProbable E3 ubiquitin-protein ligase dma1; EC 2.3.2.27; RING-type E3 ubiquitin transferase dma1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-267
Target Protein Sequence
MTKSVEGYLK EQELAAETDS EKDDDKISIR LTNFVGPNAH SFSFDPLVRY WNRKQNNLPI YIGRYTERYN GGDVSAIVFR SKVVSRRHAQ IFYENNTWYI QDMGSSSGTF LNHVRLSPPS KTSKPYPISN NDILQLGADY RGGHEVNYRC VRARVELNNS WKIKLSPYNL NEFKRMQELV LCGSSESGPP ECCICLMPVL PCQALFVAPC SHSYHYKCIR PTLNESHPYF SCFICRKYHD LEAPVEEGDE SLNDLLRNAT VKDDASE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable E3 ubiquitin-protein ligase which is a component of the spindle assembly checkpoint, required to prevent septum formation and premature exit from mitosis if spindle function is compromised. Inhibits the septation initiation netwok (SIN) during spindle checkpoint activation. The effect appears to be mediated through preventing the SIN activator, plo1 kinase, from localizing to the SPB.
Gene References into Functions
  1. Dma1 dimerization is required for proper localization at spindle pole bodies and the cell division site, E3 ligase activity, and mitotic checkpoint function PMID: 22669973
  2. Dma1 functions in parallel with the meiosis-specific Sid2-related protein kinase Slk1/Mug27 and the septation initiation network (SIN) signaling during sporulation, possibly through regulating proper forespore membrane assembly. PMID: 20980623
  3. Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. PMID: 21131906
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Note=Localizes also at the division site in mitosis.
Protein Families
DMA1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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