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Recombinant Schizosaccharomyces pombe Protein mes1 (mes1)

Recombinant Schizosaccharomyces pombe Protein mes1 (mes1)

Code	CSB-YP328203SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP328203SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP328203SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP328203SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP328203SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

mes1

Uniprot No.

P41005

Alternative Names

mes1; SPAC5D6.08c; Protein mes1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Expression Region

1-101

Target Protein Sequence

MVNTDNKENE PPNMEKAHMD SSNALYRVQR PLQRRPLQEL SIELVKPSQT ITVKKSKKST NSSSYFAQLH AASGQNPPPS VHSSHKQPSK ARSPNPLLSM R

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Specifically required for meiosis II (MII). Binds to slp1, an activator of the anapahase promoting complex/cyclcosome (APC/C), and counteracts its function in promoting proteolysis of cdc13. By suppressing the degradation of cdc13 at anaphase I this protein may help maintain a sufficient level of cdc2 kinase activity to complete MII.

Gene References into Functions

Mes1 interacts with two coactivators in a different way to control the activity of the APC/C required for the meiosis I/meiosis II transition. PMID: 21389117
Mes1 is a substrate of the APC/C as well as an inhibitor, allowing autoregulation of the APC/C in meiosis. PMID: 18331722

Subcellular Location

Cytoplasm. Nucleus.

Database Links

KEGG: spo:SPAC5D6.08c

STRING: 4896.SPAC5D6.08c.1

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