Recombinant Schizosaccharomyces pombe Replication termination factor 1 (rtf1)

Code CSB-YP892441SXV
MSDS
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Source Yeast
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Code CSB-EP892441SXV
MSDS
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Source E.coli
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Code CSB-EP892441SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892441SXV
MSDS
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Source Baculovirus
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Code CSB-MP892441SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rtf1
Uniprot No.
Alternative Names
rtf1; SPAC22F8.07c; Replication termination factor 1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-466
Target Protein Sequence
MQGKNNLSCR PDTEDNEELF VDDQLLSPIG DSKNTSSFIY LGNPISFHEY NYDETMVSPE NVKTAIAGSA KDHETCRGFK KTGTTSYKDF VFSRDYTNWT PTFWVLLSQL IDEFLKESEL NFVAARDLLI KTKRLPKPFN NLLIQFQIQV PNVSRRTVYR HLKGYFNIPG YERFQYVKKA SSGSWGANDI ITLEKEIAMF KKKKNWSDEQ FLQYVWSDNH RDEMKTLYNC LYELIDRDKK SIYNYLRRKY NPFKKKCKWT IEDEAELKKL VEKHGTSWSL IGKLSNRLPM HCRDHWRDYI QPGEINRSPW TIQEKEKLIK TVNQYLQSNP SSPIQWSLIS KNMRNRHRHH CRWKYYTLIS RDIHNSSPFK LGDSIWLIER MMDLNVAEER MIDWKCLSEY ANHLWTADAC KSHFERIKKT LFIDGLSTFS DTLIHLHKML NSSPEETYIS NLHDSYTAFS NADDLC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Mediates site-specific replication termination at the polar replication barrier RTS1, a barrier which ensures that replication of the mat1 locus in S.pombe occurs in the centromere-proximal direction.
Gene References into Functions
  1. We propose that this opposition constitutes a Cdk9 auto-regulatory mechanism, such that a positive effect on elongation, driven by the PAF pathway, is kept in check by a negative effect of Prf1/Rtf1 and downstream mono-ubiquitylation of histone H2B PMID: 24385927
  2. multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element PMID: 18723894
  3. Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin PMID: 19416828
Subcellular Location
Nucleus.
Database Links
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