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Recombinant Schizosaccharomyces pombe S-phase delaying protein 1 (spd1)

Recombinant Schizosaccharomyces pombe S-phase delaying protein 1 (spd1)

Code	CSB-YP604619SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP604619SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP604619SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP604619SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP604619SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

spd1

Uniprot No.

Q10585

Alternative Names

spd1; SPAC29B12.03; S-phase delaying protein 1; Protein p14

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Expression Region

1-124

Target Protein Sequence

MHSSKRVMTT KTHVEQPESS MRPQLPESIQ GSLMDVGMRV RKSISTGYKS KQTTFPAYNP PLYNTVSENI ALKNTAFSYE PNGTKRPFEQ AIPNYNWANP PQDFEEPEWL KPFDVVMEGT NERL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Regulates the ribonucleotide reductase activity through its mediation of the nuclear localization of suc22, the small subunit of the ribonucleotide reductase. Delays the progression of the G1-S phase transition, thereby ensuring the G1 phase is complete. Interacts with both p34 and the p34-p56 complex, although no direct inhibitory effect on the bound proteins has been demonstrated. The action of p14 may happen coincidentally with the cdc10 function or may happen downstream of this.

Gene References into Functions

The interaction of Spd1 with the polymerase processivity factor proliferating cell nuclear antigen (PCNA), complexed onto DNA. PMID: 22464192
Spd1 can interact with both R1 and R2 subunits, and show that the major restraint of RNR in vivo by Spd1 is unrelated to R2 subcellular localization PMID: 20516199

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

DIF1/spd1 family

Database Links

KEGG: spo:SPAC29B12.03

STRING: 4896.SPAC29B12.03.1

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