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Recombinant Schizosaccharomyces pombe SNF1-like protein kinase ssp2 (ssp2), partial

Recombinant Schizosaccharomyces pombe SNF1-like protein kinase ssp2 (ssp2), partial

Code	CSB-YP526724SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP526724SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP526724SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP526724SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP526724SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ssp2

Uniprot No.

O74536

Alternative Names

ssp2; SPCC74.03cSNF1-like protein kinase ssp2; EC 2.7.11.1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine protein kinase essential for release from glucose repression via the phosphorylation of scr1 upon glucose deprivation. Catalytic subunit of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes. The complex phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, leading to transcriptional activation through TBP recruitment to the promoters. Regulates proper cell cycle exit and sexual differentiation. Regulates also ste11 levels under nitrogen deprivation.

Gene References into Functions

Deletion of epe1 (+) impairs downstream events following phosphorylation of Ssp2, including nuclear translocation of Ssp2, sexual differentiation and inhibition of fatty acid synthesis. Our study reveals a novel way in which a jmjC-domain-containing protein regulates adaptive response by directly binding to a principal sensor. PMID: 28600551
results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose. PMID: 22140232
Overexpression of the gsk3+ gene or the gsk31+ gene, another GSK3 member, rescues defective growth of Deltassp2 cells at restrictive temperatures and under glucose depletion as well as sexual differentiation under nitrogen depletion. PMID: 27604537
Nitrogen-stress-induced TORC1 inhibition differs from amino-acid-dependent control of TORC1 and requires the Ssp2 (AMPKalpha) kinase. PMID: 25639242

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily

Database Links

KEGG: spo:SPCC74.03c

STRING: 4896.SPCC74.03c.1

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