Recombinant Schizosaccharomyces pombe Scavenger mRNA-decapping enzyme DcpS (nhm1)

Code CSB-YP879118SXV
MSDS
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Source Yeast
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Code CSB-EP879118SXV
MSDS
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Source E.coli
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Code CSB-EP879118SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879118SXV
MSDS
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Source Baculovirus
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Code CSB-MP879118SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nhm1
Uniprot No.
Alternative Names
nhm1; dcps; SPBP4H10.20m7GpppX diphosphatase; EC 3.6.1.59; Hint-related 7meGMP-directed hydrolase 1; Nuclear histidine triad; HIT) motif protein 1; Scavenger mRNA-decapping enzyme DcpS
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-304
Target Protein Sequence
MEESSAAKIQ LLKEFKFEKI LKDDTKSKII TLYGKIRNEV ALLLLEKTAF DLNTIKLDQL ATFLQDTKLV ENNDVFHWFL STNFQDCSTL PSVKSTLIWP ASETHVRKYS SQKKRMVCET PEMYLKVTKP FIETQRGPQI QWVENILTHK AEAERIVVED PDPLNGFIVI PDLKWDRQTM SALNLMAIVH ATDIASIRDL KYKHIPLLEN IRNKVLTEVP KQFSVDKNQL KMFVHYLPSY YHLHVHILHV DHETGDGSAV GRAILLDDVI DRLRNSPDGL ENVNITFNIG EQHFLFQPLT NMNA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Has no activity towards mRNA molecules longer than 25 nucleotides. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCS1 to m7GMP. Inhibits mRNA translation. Binds to the m7GpppG cap analog.
Subcellular Location
Cytoplasm. Nucleus. Note=Predominantly nuclear.
Protein Families
HIT family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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