Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase crk1 (crk1)

Code CSB-YP622439SXV
MSDS
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Source Yeast
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Code CSB-EP622439SXV
MSDS
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Source E.coli
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Code CSB-EP622439SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622439SXV
MSDS
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Source Baculovirus
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Code CSB-MP622439SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
crk1
Uniprot No.
Alternative Names
crk1; mcs6; mop1; SPBC19F8.07Serine/threonine-protein kinase crk1; EC 2.7.11.23; Mitotic catastrophe suppressor 6
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-335
Target Protein Sequence
MDIEKSDKWT YVKERKVGEG TYAVVFLGRQ KETNRRVAIK KIKVGQFKDG IDISALREIK FLRESRHDNV IELVDVFSTK SNLNIILEFL DSDLEMLIKD KFIVFQPAHI KSWMVMLLRG LHHIHSRFIL HRDLKPNNLL ISSDGVLKLA DFGLSRDFGT PSHMSHQVIT RWYRPPELFM GCRSYGTGVD MWSVGCIFAE LMLRTPYLPG ESDLDQLNVI FRALGTPEPE VIKSMQQLPN YVEMKHIPPP NGGMEALFSA AGHEEIDLLK MMLDYNPYRR PTAQQALEHH YFSALPKPTH PSLLPRKGGE EGIKHVSSDL QRQNNFPMRA NIKFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein kinase essential for cell proliferation, where it is required for completion of cytokinesis. Phosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II.
Gene References into Functions
  1. Cdk7 kinase modulates transcription through its RNA Pol II C-terminal domain kinase activity and also through the Cdc2-activating kinase activity. PMID: 25691663
  2. identified a novel Skp1 association with Mcs2 and Pmh1 that appears to be SCF-independent both in respect to subunit composition and absence of SCF dependent proteolysis PMID: 15555586
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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