Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase srb10 (srb10)

Code CSB-YP517601SXV
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Source Yeast
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Code CSB-EP517601SXV
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Source E.coli
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Code CSB-EP517601SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517601SXV
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Source Baculovirus
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Code CSB-MP517601SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
srb10
Uniprot No.
Alternative Names
srb10; cdk8; prk1; SPAC23H4.17c; Serine/threonine-protein kinase srb10; EC 2.7.11.22; EC 2.7.11.23; Cyclin-dependent kinase 8; Suppressor of RNA polymerase B srb10
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-369
Target Protein Sequence
MKDGYKIIGF ISSGTYGKVY KAVSSNSNDK RLFAIKKFKA ESKQVSSNAQ QTGVSQSAIR EMMLCREIQH ENIVSLVQVL LKDGTISMVF EYAEHDLLQI IHFHSRSRTR QIPPSILKSI LWQIINGVAY LHENWIMHRD LKPANIMITA TGKVKIGDLG LGRLIRDPIL PFYSSDRVVV TIWYRAPELL LGAHDYTPAI DVWAIGCIYG EMLALSPLFK GDEIKMEDKK VVPFQSTQML RIMELLGTPT EERWPGLKNY PEYYQLSSFE VRYWNNLLPQ WYQTVKNRDP QGLDLLMKML QYDPKSRITA KQALEHVFFT SDKLWTTSPF LNQPIHYPER RISEDDSEVS SKRVLSTSLR SESKRFKGN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.
Gene References into Functions
  1. The Mediator subunit cyclin-dependent kinase 8 (Cdk8) phosphorylates the forkhead 2 (Fkh2) protein in a periodic manner that coincides with gene activation during mitosis PMID: 22451489
  2. Data demonstrated that Mediator regulates Cdk8 activity and mutations in the complex can both speed up and delay entry into mitosis. PMID: 25154415
  3. The crystal structure of cyclin C is reported, and a model of the cyclin-dependent kinase 8 (CDK8)/cyclin C pair is presented. An alternative mechanism of CDK8 activation based on the CDK8/cyclin C model is discussed. [CDK8] PMID: 15979093
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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