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Recombinant Schizosaccharomyces pombe Start control protein cdc10 (cdc10), partial

Recombinant Schizosaccharomyces pombe Start control protein cdc10 (cdc10), partial

Code	CSB-YP365523SXV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP365523SXV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP365523SXV-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP365523SXV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP365523SXV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

cdc10

Uniprot No.

P01129

Alternative Names

cdc10; SPBC336.12cStart control protein cdc10

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Major component of the cell cycle transcription factor complex MBF (MCB binding factor, also known as DSC1), that controls G1-S phase specific gene expression. Involved in the control of rRNA production, via interaction with pol5. May be involved in the transcriptional regulation of the cdc22 and cdt1 genes. In fission yeast, two genes, cdc10 and cdc2, are required for the cell cycle control called start, the point early in the G1 phase at which cells become committed to the mitotic cycle.

Gene References into Functions

MBF dependent transcription remains active when Yox1 is phosphorylated and continues until cells are able to overcome this challenge PMID: 21132016
There may be an S-phase-specific inhibitor of spindle pole body maturation whose expression is under control of cdc10(+) PMID: 15385623
identified a mechanism by which Cdc10p controls rDNA gene expression, therefore linking the cell cycle with cellular growth PMID: 16816948
Results show that in the fission yeast Schizosaccharomyces pombe, the replication checkpoint regulates the entire G(1)/S transcriptional program by directly regulating Cdc10 subunit of MBF, the G(1)/S transcription factor. PMID: 18662996

Subcellular Location

Nucleus.

Database Links

KEGG: spo:SPBC336.12c

STRING: 4896.SPBC336.12c.1

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