Recombinant Schizosaccharomyces pombe Target of rapamycin complex 2 subunit bit61 (bit61)

Code CSB-YP527939SXV
MSDS
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Source Yeast
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Code CSB-EP527939SXV
MSDS
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Source E.coli
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Code CSB-EP527939SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527939SXV
MSDS
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Source Baculovirus
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Code CSB-MP527939SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bit61
Uniprot No.
Alternative Names
bit61; SPCC777.08cTarget of rapamycin complex 2 subunit bit61; TORC2 subunit bit61
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-422
Target Protein Sequence
MVGRGSFSST SSASSINWIP KNTKTSIESV SNTISLSENG QNQDLETVTT KESNVGDSDT TENIKSPFNG QWPFSRRSSQ SSSHPVFEET HWSKHSKRPG KLNVLTPTSP SNVNAEVQSI STKTQLSLLN LSPHKHKKPK DGLDLSALQK TLNGSRNFLR GRRDAGGIFG ASIPQSLVTN QIINGFGAAS LAFAKLGKVR SPLEGRFNLV PISADETWLI VESEVCSLYS GEALHYSLED LNGILILHLQ ALIRDTKMNE FVGHLETLFR KATKCLSDSL SPVPEELFLN RIIETWLFFF SSVLPYVQGV FLPIKTKLFD EQEKTQLPYE VNEFCSTNRE KLNVHRLALM TFRDYMVLPI ANRIQICIGR AESAENALDN AGEVFARLFQ ILSLLASVRT NDSKEQEITN LATKVRCLLI AS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of TORC2, which regulates multiple cellular processes to control cell growth in response to environmental signals. TORC2 is required for cell survival under various stress conditions. TORC2 positively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Positively regulates amino acid uptake through the control of expression of amino acid permeases.
Gene References into Functions
  1. Our work reveals that SAGA is a direct target of nutrient-sensing pathways and has uncovered a mechanism by which TORC1 and TORC2 converge to control gene expression and cell fate decisions. PMID: 29079657
  2. findings highlight the significance of glucose availability in regulation of TORC2-Gad8 and indicate a novel link between the cAMP/PKA, Pmk1/MAPK, and TORC2-Gad8 signaling PMID: 24928510
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
BIT61 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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