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Recombinant Schizosaccharomyces pombe Tip elongation aberrant protein Tea4 (tea4), partial

Recombinant Schizosaccharomyces pombe Tip elongation aberrant protein Tea4 (tea4), partial

Code	CSB-YP528900SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP528900SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP528900SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP528900SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP528900SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

tea4

Uniprot No.

O60132

Alternative Names

tea4; wsh3; SPBC1706.01; Tip elongation aberrant protein Tea4; Cell polarity protein tea4; Win1-interacting SH3 domain protein

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Cell polarity factor essential for the bipolar localization and function of structures containing the cell-end marker tea1 during the normal cell cycle. Regulates cell polarity in complex with tea1 and together with the stress signaling MAPK cascade, contributes to cell polarity maintenance under stress conditions. Required for the localization of for3 at the cell tip specifically during initiation of bipolar growth. During the new end take off (NETO), formation of a protein complex that includes tea1, tea4 and for3 is necessary and sufficient for the establishment of cell polarity and localized actin assembly at new cell ends.

Gene References into Functions

Tea4-PP1 landmark promotes local Cdc42 activation and growth both through Cdc42 GEF recruitment and by creating a local trough in a Cdc42 GAP. PMID: 24554432
Tea4/Wsh3, an essential component of the Tea1 cell-end complex, is required for bipolar distribution of Tea1 and contributes to the maintenance of cell polarity under stress conditions. [tea4] PMID: 15936270
study reports distribution of the 2 PP1 isoforms (dis2 & Sds21)in fission yeast to new subcellular locations & demonstrates that recruitment of Dis2 to cell tips by Wsh3/Tea4 is important for the regulation of the microtubule-mediated polarised tip growth PMID: 17895368

Subcellular Location

Cytoplasm, cytoskeleton. Note=Through it's binding with tea1, is transported by the cytoplasmic microtubule system and is localized at cell tips, microtubule plus ends and cytoplasmic dots.

Database Links

KEGG: spo:SPBC1706.01

STRING: 4896.SPBC1706.01.1

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