Recombinant Schizosaccharomyces pombe Uncharacterized protein C16E9.01c (SPBC16E9.01c, SPBP16F5.09c)

Code CSB-YP515671SXV
MSDS
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Source Yeast
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Code CSB-EP515671SXV
MSDS
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Source E.coli
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Code CSB-EP515671SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP515671SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP515671SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
php4
Uniprot No.
Alternative Names
php4; SPBC16E9.01c; SPBP16F5.09c; CCAAT-binding factor complex subunit php4
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-295
Target Protein Sequence
MESSKSPSEV EKSSSASPAP QKPMIRVSKQ WVVPPRPKPG RKPALDALGR RKAPIKPRPG PTSALSVEEA KFRVREKQYQ DTIGKLQKEN NELLEQLEML QAQLKNSTLD SPKEVEVNSE VVKPDSATTE NENRYVNQYN YPVEPPCAKN AVYTEIPIEL DPHAFLGDSA KRIRVDSDSK DAKSVPSENG RIRVSMSPQN EINFTPENPA VMEKIRKRGV CNSVEGCLYS GSPKSVKRVR ESEETKVYAQ LLIDLHKSSK SAPMLKAGPS IAFKLPTMEP NFNDVRPVTS ISSSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the transcription regulatory CCAAT-binding complex. Required for the reprogramming of the cell for iron use. Downregulates pcl1, sdh4, and isa1 underlow-iron conditions.
Gene References into Functions
  1. Study aimed to biochemically define these protein-protein and protein-metal interactions. Grx4 was found to bind a [2Fe-2S] cluster with spectroscopic features similar to other CGFS glutaredoxins. Grx4 and Php4 also copurify as a complex with a [2Fe-2S] cluster that is spectroscopically distinct from the cluster on Grx4these results show that Grx4 controls Php4 function through binding of a bridging [2Fe-2S] cluster. PMID: 28725905
  2. Grx4 independently governs the Php4 and Fep1 repressors through metal transfer. total or partial disassembly of the Grx4-Fra2 cluster actively participates in iron-containing Fep1 activation PMID: 25806539
  3. Grx4 and Crm1 are novel components involved in the mechanism by which Php4 is inactivated by iron in a Fep1-independent manner. PMID: 19502236
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle pole.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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