Recombinant Schizosaccharomyces pombe Uncharacterized protein C1919.03c (SPCC1919.03c)

Code CSB-YP302907SXV
MSDS
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Source Yeast
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Code CSB-EP302907SXV
MSDS
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Source E.coli
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Code CSB-EP302907SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP302907SXV
MSDS
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Source Baculovirus
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Code CSB-MP302907SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
amk2
Uniprot No.
Alternative Names
amk2; SPCC1919.03c5'-AMP-activated protein kinase subunit beta; AMPK subunit beta
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-298
Target Protein Sequence
MGNVQSQEGE TRAHAVPSQD ATTTPDNANN VPKEPRAQSM ISIAADDLNQ EGEMSDDNQQ EGGNNRTSQN GTSGSSGHTK RRSQTSGKKT HQPYSGPCVP TIIRWRGGGE VVYVTGSFSR WKKKIQLLKS EDYTVLLQLR PGTQRFKFLV DGIWCCSSDF PTATDAEGNL YNYLEVEANE KLGASIDERL SQVHTDLPME EKSESEQYST EIPAFLTSNT LQELKLPKPP SLPPHLEKCI LNSNTAYKED QSVLPNPNHV LLNHLAAANT QLGVLALSAT TRYHRKYVTT AMFKNFDV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Beta subunit of AMP-activated protein kinase (AMPK), which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the AMPK kinase complex.
Gene References into Functions
  1. Data indicate that AMP-activated protein kinase beta subunit Amk2 (Amk2) and glycogen synthase kinase 3 (Gsk3) act in parallel to inhibit TORC1 (Tor2) function. PMID: 28281664
  2. crystal structures of a heterotrimeric regulatory core fragment from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide PMID: 17937917
Subcellular Location
Cytoplasm.
Protein Families
5'-AMP-activated protein kinase beta subunit family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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