Recombinant Schizosaccharomyces pombe Zinc finger protein zfs1 (zfs1)

Code CSB-YP344496SXV
MSDS
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Source Yeast
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Code CSB-EP344496SXV
MSDS
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Source E.coli
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Code CSB-EP344496SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344496SXV
MSDS
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Source Baculovirus
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Code CSB-MP344496SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
zfs1
Uniprot No.
Alternative Names
zfs1; moc4; SPBC1718.07cZinc finger protein zfs1; Multicopy suppressor of overexpressed cyr1 protein 4
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-404
Target Protein Sequence
MVYSPMSRPQ VPLAFRQWPP YNYKSDPLVN SKLSQSTTSV NAGPSLISPS FLDSYANSSS LLHKPSTNLG SLKTSSLLAS DEVFPSSVMP QLRPLDSSLS VSPEMDGWPW HHNSSSNPQG YAWTPSLLSS NATSYLHSGS SPHGNTSNHP SPISSLESLP SRSSTGSGSL DFSGLANLHD DSKSLAMSLN MAGVPVSVDE NSQTSFPFVH GQPESTMSRK PKLCVQSKSM TNIRNSVAKP SLVRQSHSAG VIPIKPTASN ASIRNAPSNL SKQFSPSGNS PLTEASKPFV PQPSAAGDFR QAKGSASHPH GSGSSNGVAP NGKRALYKTE PCKNWQISGT CRYGSKCQFA HGNQELKEPP RHPKYKSERC RSFMMYGYCP YGLRCCFLHD ESNAQKSATI KQSP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. Binds to ARE present in the arz1 mRNA and stimulates the rate of arz1 mRNA decay. Required for coordination of septum formation with exit from mitosis. Involved in the mating response pathway. Induces sexual development and ascus formation.
Gene References into Functions
  1. We also found that Zfs1 undergoes phosphorylation, which is stimulated upon nitrogen depletion or inhibition of the TOR pathway. Phosphorylation of Zfs1 modulates accumulation of Puc1 and plays an important role in the response of the cell to sexual differentiation signals. PMID: 29084823
  2. The data suggested that Zfs1p can both directly and indirectly regulate the levels of transcripts involved in cell-cell adhesion. PMID: 22907753
  3. zfs1 and its targets represent a useful model system for studies of zinc finger protein/AU-rich element interactions that result in mRNA decay. PMID: 18042546
Subcellular Location
Cytoplasm. Nucleus.
Database Links
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