Recombinant Sex-determining transformer protein 2 (tra-2), partial

Code CSB-YP327358CXY
MSDS
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Source Yeast
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Code CSB-EP327358CXY
MSDS
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Source E.coli
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Code CSB-EP327358CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327358CXY
MSDS
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Source Baculovirus
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Code CSB-MP327358CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tra-2
Uniprot No.
Alternative Names
tra-2; C15F1.3; Sex-determining transformer protein 2; Ce-Tra-2
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a major role in controlling sexual cell fates. Promotes female development in XX animals where it sequesters one or more of the FEM proteins to the membrane thereby freeing the tra-1 protein (a putative transcription factor) to enter the nucleus and promote female development. In XO animals it acts as a receptor for her-1 which prevents it from binding to FEM proteins thereby repressing the activity of tra-1. Negatively regulates male development when bound to fem-3 and is required together with tra-1 for promoting spermatogenesis. Also required for feminizing tra-3 activity.
Gene References into Functions
  1. Tra-2 mRNA export is highly regulated and plays an important role in development by regulating the activity of tra-2 mRNA in the cytoplasm. PMID: 15175155
Subcellular Location
Membrane; Multi-pass membrane protein.
Tissue Specificity
Somatic and germline tissues. Isoform Tra-2B is specific to oocytes.
Database Links

KEGG: cel:CELE_C15F1.3

STRING: 6239.C15F1.3a

UniGene: Cel.18385

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