Recombinant Spindle assembly abnormal protein 5 (sas-5)

Code CSB-YP632359CXY
MSDS
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Source Yeast
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Code CSB-EP632359CXY
MSDS
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Source E.coli
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Code CSB-EP632359CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP632359CXY
MSDS
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Source Baculovirus
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Code CSB-MP632359CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sas-5
Uniprot No.
Alternative Names
sas-5; F35B12.5; Spindle assembly abnormal protein 5
Species
Caenorhabditis elegans
Expression Region
1-404
Target Protein Sequence
MNNYDDLPCS IYFKKPTVQE FVDQPRVFED SEVPAFQEVL QLPQERTKPP VPSTQPIVAA VEVAKKKSCL SAPKPRKEPP SHPALRQKTV AFGKTVNVSQ TVEGTSRNSK KVLASTMSAQ NTTTTEEQAA ENWRDAMKTE LQTIRTEIQE ETARRQEELN AQNLVKMQEL MSNFFQKITI PKQQAIEPVE KDKENFHESP RQSRQQKPAS KIASAREVIK RDGVIPPEAL TIIEQRLRSD PMFRQQIDNV LADAECDANR AAYSPPPPMS EVRYGSGVNP ALMRETLTVE RSIRYDNGLA SIDSRQWTNE RRDNRAPDSY RTYEPDQPCH SLYQKGQSIS YYPSEAAGKT TARNNRTGYY VEDSSDHEED VVVNKRGQNY HEQAVPETPA ERERRIREKY ARRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.
Gene References into Functions
  1. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo. PMID: 26023830
  2. SAS-5 has a role in nuclear morphology in Caenorhabditis elegans PMID: 15724817
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Localizes to centrioles. Shuttles between centrioles and the cytoplasm throughout the cell cycle. Requires zyg-1 and dephosphorylation by the PP2A complex for centriole localization.
Database Links

KEGG: cel:CELE_F35B12.5

STRING: 6239.F35B12.5a

UniGene: Cel.3763

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