Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

clfB

Uniprot No.

Q6GDH2

Research Area

Others

Alternative Names

clfB; SAR2709Clumping factor B; Fibrinogen receptor B; Fibrinogen-binding protein B

Species

Staphylococcus aureus (strain MRSA252)

Source

E.coli

Expression Region

45-542aa

Target Protein Sequence

SEQSNDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKTMSTQTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATNSELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDKVTASDFKLEKTAFDPNQSGNTFMAANFKVTGQVKSGDYFTAKLPDSVTGNGDVDYSNSNNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKAPKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTGEFKDKISYKYDNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQENIDPATGKDYSIFGWNNENVVRYGGGSADGDSAVN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

69.8kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Staphylococcus aureus (strain MRSA252) clfB contains amino acids 45-542. The expected molecular weight for the clfB protein is calculated to be 69.8 kDa. The clfB protein was expressed in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the clfB encoding gene fragment was conducted, allowing for easier detection and purification of the clfB protein in subsequent stages.

Staphylococcus aureus clumping factor B (ClfB) is a microbial surface component recognizing adhesive matrix molecules (MSCRAMM) crucial for bacterial adhesion and biofilm formation. The main function of ClfB involves binding to fibrinogen, facilitating S. aureus colonization on host tissues and medical implants. In infectious disease research, ClfB is a virulence factor and a potential target for therapeutic interventions against S. aureus infections. Investigating ClfB provides insights into bacterial pathogenesis, biofilm formation, and host-pathogen interactions. Additionally, understanding ClfB's role in biofilm-related infections contributes to antimicrobial strategies and the development of novel therapeutics to combat S. aureus-associated diseases, impacting medical and microbiological research.

Target Background

Function

Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps.

Subcellular Location

Secreted, cell wall; Peptidoglycan-anchor.

Protein Families

Serine-aspartate repeat-containing protein (SDr) family

Database Links

KEGG: sar:SAR2709

Code	CSB-EP753442SKV
Abbreviation	Recombinant Staphylococcus aureus clfB protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP753442SKV could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain MRSA252) clfB. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP753442SKV could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain MRSA252) clfB.
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Recombinant Staphylococcus aureus Clumping factor B (clfB), partial

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