Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

fabI

Uniprot No.

Q6GI75

Research Area

Others

Alternative Names

fabI; SAR0978Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI; ENR; EC 1.3.1.39; NADPH-dependent enoyl-ACP reductase

Species

Staphylococcus aureus (strain MRSA252)

Source

E.coli

Expression Region

1-256aa

Target Protein Sequence

MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERSRKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDLRGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVMGVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLKRNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

44.0kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Staphylococcus aureus (strain MRSA252) fabI contains amino acids 1-256. The theoretical molecular weight of the fabI protein is 44 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal 6xHis-SUMO tag into the fabI encoding gene fragment was conducted, allowing for easier detection and purification of the fabI protein in subsequent stages.

Staphylococcus aureus Enoyl-[acyl-carrier-protein] Reductase [NADPH] FabI is a key enzyme in bacterial fatty acid biosynthesis. Its main function involves catalyzing the reduction of enoyl-ACP, a critical step in fatty acid elongation. FabI is essential for bacterial membrane biogenesis, making it a target for antibiotic research. Investigating FabI provides insights into bacterial physiology, antimicrobial resistance, and antibiotic development. Understanding its structure and mechanism aids in designing novel antibacterial agents. FabI research extends to the broader field of bacterial metabolism, offering potential applications in developing strategies to combat Staphylococcus aureus infections and addressing challenges related to antibiotic resistance in clinical settings.

Target Background

Function

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family, FabI subfamily

Database Links

KEGG: sar:SAR0978

Code	CSB-EP750140SKV
Abbreviation	Recombinant Staphylococcus aureus fabI protein
MSDS	MSDS Datasheet
Size	US$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP750140SKV could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain MRSA252) fabI. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP750140SKV could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain MRSA252) fabI.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Staphylococcus aureus Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (fabI)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage