Recombinant Staphylococcus aureus Probable transglycosylase isaA (isaA)

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Code CSB-EP350770SUL
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
isaA
Uniprot No.
Research Area
Cell Biology
Alternative Names
isaA; MW2490Probable transglycosylase IsaA; EC 3.2.-.-; Immunodominant staphylococcal antigen A
Species
Staphylococcus aureus (strain MW2)
Source
E.coli
Expression Region
30-233aa
Target Protein Sequence
AEVNVDQAHLVDLAHNHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADYTTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSSAAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINAAVKAYKAQGLGAWGF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
28.8 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

In the general approach to express the recombinant Staphylococcus aureus (strain MW2) isaA protein, a plasmid encoding the Staphylococcus aureus (strain MW2) isaA protein (30-233aa) is first constructed. The constructed plasmid is then introduced into e.coli cells. Plasmid-containing e.coli cells are screened and cultured under conditions that induce the protein expression. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. Lysing the cultured cells and purifying the resulting recombinant isaA protein through affinity purification. The SDS-PAGE analysis is conducted to confirm the presence of the recombinant isaA protein and assess its purity. Its purity is over 85%.

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Target Background

Function
Is able to cleave peptidoglycan.
Subcellular Location
Secreted.
Protein Families
Transglycosylase family, IsaA subfamily
Database Links

KEGG: sam:MW2490

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