Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

sdrE

Uniprot No.

Q932F7

Research Area

Others

Alternative Names

sdrE; SAV0563Serine-aspartate repeat-containing protein E

Species

Staphylococcus aureus (strain Mu50 / ATCC 700699)

Source

E.coli

Expression Region

53-601aa

Target Protein Sequence

AENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

64.5kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Staphylococcus aureus sdrE was expressed with the amino acid range of 53-601. This sdrE protein is theoretically predicted to have a molecular weight of 64.5 kDa. This sdrE protein is produced using e.coli expression system. The N-terminal 6xHis tag was fused into the coding gene segment of sdrE, making it easier to detect and purify the sdrE recombinant protein in the later stages of expression and purification.

Staphylococcus aureus serine-aspartate repeat-containing protein E (SdrE) serves as a multifaceted virulence factor in bacterial pathogenesis. Its primary function involves mediating host-pathogen interactions by binding to host extracellular matrix proteins. In microbiology and infectious disease research, studying SdrE provides insights into Staphylococcus aureus adhesion and immune evasion mechanisms. SdrE's role in biofilm formation and immune subversion implicates it in antimicrobial resistance and vaccine development efforts. Investigating SdrE spans diverse research areas, contributing to our understanding of bacterial pathogenicity and offering potential targets for therapeutic interventions, particularly in combating Staphylococcus aureus infections and addressing antibiotic resistance challenges.

Target Background

Function

Cell surface-associated calcium-binding protein which plays an important role in adhesion and pathogenesis. Contributes to the resistance to killing by innate immune components in blood and thus attenuates bacterial clearance by interacting with host complement factor H/CFAH and modulating its activity. Inhibits also bacterial opsonization and killing by interacting with host complement regulator C4BPA and thus inhibiting classical complement pathway activation.

Subcellular Location

Secreted, cell wall; Peptidoglycan-anchor.

Protein Families

Serine-aspartate repeat-containing protein (SDr) family

Database Links

KEGG: sav:SAV0563

STRING: 158878.SAV0563

Code	CSB-EP846305SKX
Abbreviation	Recombinant Staphylococcus aureus sdrE protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP846305SKX could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain Mu50 / ATCC 700699) sdrE. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP846305SKX could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus (strain Mu50 / ATCC 700699) sdrE.
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Recombinant Staphylococcus aureus Serine-aspartate repeat-containing protein E (sdrE), partial

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