Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
sdrE; SAV0563Serine-aspartate repeat-containing protein E
Species
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Expression Region
53-601aa
Target Protein Sequence
AENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Staphylococcus aureus sdrE was expressed with the amino acid range of 53-601. This sdrE protein is theoretically predicted to have a molecular weight of 64.5 kDa. This sdrE protein is produced using e.coli expression system. The N-terminal 6xHis tag was fused into the coding gene segment of sdrE, making it easier to detect and purify the sdrE recombinant protein in the later stages of expression and purification.
Staphylococcus aureus serine-aspartate repeat-containing protein E (SdrE) serves as a multifaceted virulence factor in bacterial pathogenesis. Its primary function involves mediating host-pathogen interactions by binding to host extracellular matrix proteins. In microbiology and infectious disease research, studying SdrE provides insights into Staphylococcus aureus adhesion and immune evasion mechanisms. SdrE's role in biofilm formation and immune subversion implicates it in antimicrobial resistance and vaccine development efforts. Investigating SdrE spans diverse research areas, contributing to our understanding of bacterial pathogenicity and offering potential targets for therapeutic interventions, particularly in combating Staphylococcus aureus infections and addressing antibiotic resistance challenges.