Recombinant Thermus thermophilus 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (TTHA1582)

Code CSB-YP722518TNT
MSDS
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Source Yeast
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Code CSB-EP722518TNT
MSDS
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Source E.coli
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Code CSB-EP722518TNT-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP722518TNT
MSDS
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Source Baculovirus
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Code CSB-MP722518TNT
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
TTHA1582
Uniprot No.
Alternative Names
TTHA15828-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase; AONS/AKB ligase; EC 2.3.1.29; EC 2.3.1.47; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; Glycine acetyltransferase
Species
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Expression Region
1-395
Target Protein Sequence
MSLDLRARVR EELERLKREG LYISPKVLEA PQEPVTRVEG REVVNLASNN YLGFANHPYL KEKARQYLEK WGAGSGAVRT IAGTFTYHVE LEEALARFKG TESALVLQSG FTANQGVLGA LLKEGDVVFS DELNHASIID GLRLTKATRL VFRHADVAHL EELLKAHDTD GLKLIVTDGV FSMDGDIAPL DKIVPLAKKY KAVVYVDDAH GSGVLGEKGK GTVHHFGFHQ DPDVVQVATL SKAWAGIGGY AAGARELKDL LINKARPFLF STSHPPAVVG ALLGALELIE KEPERVERLW ENTRYFKREL ARLGYDTLGS QTPITPVLFG EAPLAFEASR LLLEEGVFAV GIGFPTVPRG KARIRNIVTA AHTKEMLDKA LEAYEKVGKR LGIIR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. It also converts 2-amino-3-ketobutyrate and CoA to glycine and acetyl-CoA. Activity is also observed with the following combinations of substrates: acetyl-CoA and either L-alanine or L-serine, pimeloyl-CoA and either glycine or L-serine, and palmitoyl-CoA with L-alanine.
Protein Families
Class-II pyridoxal-phosphate-dependent aminotransferase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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