Recombinant Transcription factor elt-1 (elt-1)

Code CSB-YP329821CXY
MSDS
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Source Yeast
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Code CSB-EP329821CXY
MSDS
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Source E.coli
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Code CSB-EP329821CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329821CXY
MSDS
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Source Baculovirus
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Code CSB-MP329821CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
elt-1
Uniprot No.
Alternative Names
elt-1; W09C2.1Transcription factor elt-1
Species
Caenorhabditis elegans
Expression Region
1-416
Target Protein Sequence
MDYEGKPVEF TLGTSSGGAS LAPTSSTTAA SIAPFSYNTS ATNYYNTTPS SYPMFLNYQY AGGTTVTTDM DAFSGMDMSM NNGVFGTQNN PSYFYPTTQL NTYGYDTLAA ATTASGITVN NNQLNVNIVQ GNGTIVPQPI TQNIISTVSN VQSSVPINNS QPLTPTGLAG CSTSSGSSSA SSSSANSTST PKNTISKANR SSGGANNSQF STEDRECVNC GVHNTPLWRR DGSGNYLCNA CGLYFKMNHH ARPLVKPKKR QQNAQKRTGI ECVNCRTNTT TLWRRNGEGH PVCNACGLYF KLHKVERPIT MKKDGIQTRN RKLSAKGSRR MKKENGGTPT SMGMPTTSSS ISSGIELDQS GVWGMKNTQP MLMTPTAYAF PASNFYFNSI EDQLEYKTCP PMMVDFGGQM KNLNLN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3' and variations thereof. During embryonic development, required for specification of cell fate of major hypodermal (epidermal) cells at the blastomere stage. The requirement is true for all four lineages derived from ABarp, ABpra and C blastomeres. Required for seam cell maintenance in late embryogenesis, for proper formation of dauer larvae and locomotion. Regulates expression of bro-1, a regulator of seam cell proliferation, via a GATA-like binding motif. Probably represses expression of eff-1 to prevent fusion of seam cells with the hypodermal syncytium. Involved in regulating expression of elt-3, a probable downstream target in hypodermal development pathways, in dorsal and ventral hypodermal cells. During postembryonic development, has a role in sperm development. Specifically, binds the bipartite consensus sequence 5'-AGATCTx(8)GATAA-3' found in genes with sperm-specific expression. Furthermore, regulates timing of developmental steps, possibly in parallel with daf-12, by controlling the expression of the let-7 family of miRNAs which in turn determines the transcription of heterochronic genes such as lin-41.
Gene References into Functions
  1. this work suggest that the contribution of ELT-1 to the control of let-7 family microRNA expression is likely through direct transcription regulation. PMID: 25816370
  2. Data suggest that ELT-1 has dual roles in the stem-like seam cells, acting both to promote proliferation and prevent differentiation. PMID: 21829390
  3. elt-1 is a key regulator of neuronal function in larvae and adult worms. PMID: 16303852
  4. analysis of regulation of sperm gene expression by the GATA factor ELT-1 PMID: 19591818
Subcellular Location
Nucleus. Perikaryon. Cell projection, axon.
Tissue Specificity
Expressed in ventral cord and retrovesicular ganglion neurons. Expressed in vulval muscles of hermaphrodites and lateral seam and tail seam cells of males. Expressed in spermatozoa within the spermatheca of hermaphrodites.
Database Links

STRING: 6239.W09C2.1a

UniGene: Cel.7646

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