Recombinant Triticum aestivum Granule-bound starch synthase 1,chloroplastic/amyloplastic (WAXY), partial

Amino acids 79-349 constitute the expression domain of recombinant Wheat WAXY. This WAXY protein is expected to have a theoretical molecular weight of 35.8 kDa. This WAXY recombinant protein is manufactured in e.coli. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of WAXY, which enables a simple process of detecting and purifying the WAXY recombinant protein in the following steps.

Granule-bound starch synthase 1 (WAXY) in Triticum aestivum, commonly known as wheat, is a chloroplastic/amyloplastic enzyme involved in starch biosynthesis. WAXY plays a crucial role in converting glucose into starch, a complex polysaccharide that serves as a major storage form of energy in plants. WAXY is responsible for synthesizing the granule-bound fraction of starch, which constitutes a significant portion of the starch granule. In chloroplasts, WAXY contributes to starch synthesis during photosynthesis, converting the sugars produced by the plant into starch for storage. In amyloplasts, WAXY is involved in starch accumulation in storage organs like seeds and tubers. The enzyme catalyzes the elongation of starch chains by adding glucose units, leading to the formation of amylose and amylopectin, the two main components of starch. Understanding the function of WAXY is crucial for improving crop yield and quality in wheat and other cereal crops. Genetic modifications targeting WAXY expression can potentially enhance starch content, alter starch composition, and influence the nutritional and processing properties of wheat grains.