Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
WAXY; Granule-bound starch synthase 1; chloroplastic/amyloplastic; EC 2.4.1.242; Granule-bound starch synthase I; GBSS-I
Species
Triticum aestivum (Wheat)
Expression Region
79-349aa
Target Protein Sequence
LVFVGAEMAPWSKTGGLGDVLGGLPAAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPCFLEKVRGKTKEKIYGPDAGTDYEDNQQRFSLLCQAALEVPRILDLNNNPHFSGPYAMLCRAVPRRAGEDVVFVCNDWHTGLLACYLKSNYQSNGIYRTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCELDNIMR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 79-349 constitute the expression domain of recombinant Wheat WAXY. This WAXY protein is expected to have a theoretical molecular weight of 35.8 kDa. This WAXY recombinant protein is manufactured in e.coli. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of WAXY, which enables a simple process of detecting and purifying the WAXY recombinant protein in the following steps.
Granule-bound starch synthase 1 (WAXY) in Triticum aestivum, commonly known as wheat, is a chloroplastic/amyloplastic enzyme involved in starch biosynthesis. WAXY plays a crucial role in converting glucose into starch, a complex polysaccharide that serves as a major storage form of energy in plants. WAXY is responsible for synthesizing the granule-bound fraction of starch, which constitutes a significant portion of the starch granule. In chloroplasts, WAXY contributes to starch synthesis during photosynthesis, converting the sugars produced by the plant into starch for storage. In amyloplasts, WAXY is involved in starch accumulation in storage organs like seeds and tubers. The enzyme catalyzes the elongation of starch chains by adding glucose units, leading to the formation of amylose and amylopectin, the two main components of starch. Understanding the function of WAXY is crucial for improving crop yield and quality in wheat and other cereal crops. Genetic modifications targeting WAXY expression can potentially enhance starch content, alter starch composition, and influence the nutritional and processing properties of wheat grains.
