Recombinant Trypanosoma cruzi Cruzipain

In Stock
Code CSB-EP333107TQY
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
N/A
Uniprot No.
Research Area
Others
Alternative Names
Cruzipain; EC 3.4.22.51; Cruzaine; Major cysteine proteinase
Species
Trypanosoma cruzi
Source
E.coli
Expression Region
123-467aa
Target Protein Sequence
APAAVDWRARGAVTAVKDQGQCGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDKTDSGCSGGLMNNAFEWIVQENNGAVYTEDSYPYASGEGISPPCTTSGHTVGATITGHVELPQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTSCVSEQLDHGVLLVGYNDSAAVPYWIIKNSWTTQWGEEGYIRIAKGSNQCLVKEEASSAVVGGPGPTPEPTTTTTTSAPGPSPSYFVQMSCTDAACIVGCENVTLPTGQCLLTTSGVSAIVTCGAETLTEEVFLTSTHCSGPSVRSSVPLNKCNRLLRGSVEFFCGSSSSGRLADVDRQRRHQPYHSRHRRL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
43.9 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Enhance your study on parasitic diseases with our Recombinant Trypanosoma cruzi Cruzipain. This product features the protein Cruzipain, also known as Cruzaine, the major cysteine proteinase in Trypanosoma cruzi. The enzyme plays a crucial role in the life cycle of the parasite, especially in its interactions with host organisms, making it a fascinating target for parasitology and infectious disease research.

This recombinant Cruzipain covers the full length of the mature protein, specifically the amino acid region from 123-467. It is expressed in E.coli and carries N-terminal 10xHis and C-terminal Myc tags, facilitating convenient purification and detection. The Recombinant Trypanosoma cruzi Cruzipain shows a purity greater than 85% as determined by SDS-PAGE, ensuring the high quality of the protein. It comes in either a liquid form or a lyophilized powder, adaptable to your experimental needs. This product opens up possibilities for further understanding the pathogenesis of Chagas disease and the exploration of potential therapeutic targets.

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Target Background

Function
Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group.; The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.
Protein Families
Peptidase C1 family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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