Recombinant Tryptophan 2,3-dioxygenase (C28H8.11)

Code CSB-YP023351CXY
MSDS
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Source Yeast
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Code CSB-EP023351CXY
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Source E.coli
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Code CSB-EP023351CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023351CXY
MSDS
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Source Baculovirus
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Code CSB-MP023351CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tdo-2
Uniprot No.
Alternative Names
tdo-2; C28H8.11; Tryptophan 2,3-dioxygenase; TDO; EC 1.13.11.11; Tryptamin 2,3-dioxygenase; Tryptophan oxygenase; TO; TRPO; Tryptophan pyrrolase; Tryptophanase
Species
Caenorhabditis elegans
Expression Region
1-403
Target Protein Sequence
MACPYLGSGE LTHRVTFMEG GEECQQGVNK VEMGFGQTYS EYLQLDKILT AQRLKSEADG QRVDDEHLFI VIHQAHELWF KQIIFDLDNV RKLLNNTIVD ETKTLKIVSG LDRMTKILSL LTEQITLLDT MSPLDFVDFR KYLTPASGFQ SLQFRVLENK LGVRQERRIK YNAQHYKNVF NDTDLKTLNV TEEEKSLLTL IESWLERTPG LKSTSEDEGF WIKYEKSVNK YLADLAKQAA DPSNTEEIAK QLTAEYHKTA DAFQSILDPR QHEQHIRNGN RLLSHDATKG AMMIYFYRDM PRFSQPYQIL TFLMDIDSLF TKWRYNHVLL VQRMLGAKQG TGGSSGYMYL RSTVSDRYKV FLDLFNLSTW LIPREYIPML SPRMVKTLSE HSNLSHSQSS ESD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety. Involved in regulation of protein homeostasis, longevity and reproducive life span. Specifically regulates proteotoxicity due to age-related aggregation of proteins like alpha-synuclein, via its effects on tryptophan metabolism.
Gene References into Functions
  1. Loss of TDO-2's enzymatic activity in three amino acids deletion mutants was accompanied by an increase in motility during aging and a prolonged lifespan, which is in line with the previously observed phenotypes induced by a knockdown of the full enzyme. PMID: 27995966
  2. We identify tryptophan 2,3-dioxygenase (tdo-2), the first enzyme in the kynurenine pathway of tryptophan degradation, as a metabolic regulator of age-related alpha-synuclein toxicity. PMID: 22927396
Protein Families
Tryptophan 2,3-dioxygenase family
Tissue Specificity
Expressed in body wall muscle cells, hypodermis, PLM neurons and touch-receptor neurons.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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