Recombinant Uncharacterized protein C02F5.6 (C02F5.6)

Code CSB-YP327306CXY
MSDS
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Source Yeast
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Code CSB-EP327306CXY
MSDS
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Source E.coli
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Code CSB-EP327306CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327306CXY
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP327306CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
C02F5.6
Uniprot No.
Alternative Names
henn-1; C02F5.6; Small RNA 2'-O-methyltransferase; EC 2.1.1.n8; HEN1 methyltransferase homolog
Species
Caenorhabditis elegans
Expression Region
1-450
Target Protein Sequence
MAHTSDGWGA PYDNQTYVEA YEQLEIALLE PLDRILETAN VEEFRPKFKN HQDPNDRKNK KNNDEEWRDS IYNIATDESD TDDHEQRKNF FQPPLQVQRN SFVKNTLMEF KRSSQIDISR LAVMGCGEMS LEKGICEYLG SFGTINVLSV DIDEPSLSIG QQLLGKAESI FLRKHLERNA EILAVETGLP VLMRSYVGDI LEPDHRFADV DAIVSMEVVE HIPLPNAKKF VENVLGTLMP RIFIFSTPNH EYNAVFGMEP GEFRHGDHKF EMNRKEFSNW LEELSIRFPH YQIDPPHYIG MTRGYENLSG ASQAAVCRLQ VDLNTTLPQE VTPYEMVGHL PCRLGSRLIA YNLVKEAFLD WLEKIELQEH EPRTDGYSPY WIFNVQNILH HLKAPVSFAL TIDEKVAIKY IQGMTSRKVH AEYSHGFNGI VILQMHSKEE LIKTVQDNTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Methyltransferase that adds a 2'-O-methyl group at the 3'-end of PIWI-interacting RNAs (piRNAs) and small interfering RNAs (siRNAs) which are classes of regulatory RNAs that are involved in gene silencing in endogenous RNA interference (RNAi) pathways. Methylation protects the 3'-end of small RNAs from tailing and trimming and could constitute a recognition signal for appropriate argonaute machineries (Probable). Methylates and stabilizes 26G-siRNAs (a class of 26 nucleotide siRNAs that possess a monophosphorylated guanine residue at the 5'-end) when they are bound by argonaute protein ergo-1. This occurs in the female germline and embryo, but not in the male germline. Does not methylate 26G-siRNAs bound by argonaute proteins alg-3 or alg-4. Methylates and stabilizes 21U-piRNAs, which are a class of 21 nucleotide piRNAs that possess a uracil residue at the 5'-end, in the male and female germline. In addition, may play a role in exogenous RNAi (exoRNAi) pathways in the germline.
Subcellular Location
Cytoplasm. Nucleus, nucleoplasm. Cytoplasmic granule.
Tissue Specificity
Broadly expressed in the germline and somatic tissues in both hermaphrodites and males.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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