Purity
Greater than 85% as determined by SDS-PAGE.
Species
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Expression Region
354-808aa
Target Protein Sequence
IERILVEDVFLDRRASSKTHKYGPRWNSVYALPYNAGKMYVQHIPGFYDVSLRAVGQGTAIWHHMILSTAACAISNRISHGDGLGFLLDAAIRISANCIFLGRNDNFGVGDPCWLEDHLAGLPREAVPDVLQVTQLVLPNRGPTVAIMRGFFGALAYWPELRIAISEPSTSLVRYATGHMELAEWFLFSRTHSLKPQFTPTEREMLASFFTLYVTLGGGMLNWICRATAMYLAAPYHSRSAYIAVCESLPYYYIPVNSDLLCDLEVLLLGEVDLPTVCESYATIAHELTGYEAVRTAATNFMIEFADCYKESETDLMVSAYLGAVLLLQRVLGHANLLLLLLSGAALYGGCSIYIPRGILDAYNTLMLAASPLYAHQTLTSFWKDRDDAMQTLGIRPTTDVLPKEQDRIVQASPIEMNFRFVGLETIYPREQPIPSVDLAENLMQYRNEILGLDW
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
In expressing the recombinant Varicella-zoster virus (strain Dumas) ORF11 protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (354-808aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. The recombinant Varicella-zoster virus (strain Dumas) ORF11 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.
