Recombinant Varicella-zoster virus Transcriptional transactivator IE63 (ORF63)

Code CSB-YP758863VAQ
MSDS
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Source Yeast
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Code CSB-EP758863VAQ
MSDS
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Source E.coli
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Code CSB-EP758863VAQ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP758863VAQ
MSDS
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Source Baculovirus
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Code CSB-MP758863VAQ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ORF63;
Uniprot No.
Alternative Names
ORF63;; ORF70; Transcriptional regulator ICP22 homolog; Immediate-early protein 63; IE63; Transcriptional transactivator IE63
Species
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Expression Region
1-278
Target Protein Sequence
MFCTSPATRG DSSESKPGAS VDVNGKMEYG SAPGPLNGRD TSRGPGAFCT PGWEIHPARL VEDINRVFLC IAQSSGRVTR DSRRLRRICL DFYLMGRTRQ RPTLACWEEL LQLQPTQTQC LRATLMEVSH RPPRGEDGFI EAPNVPLHRS ALECDVSDDG GEDDSDDDGS TPSDVIEFRD SDAESSDGED FIVEEESEES TDSCEPDGVP GDCYRDGDGC NTPSPKRPQR AIERYAGAET AEYTAAKALT ALGEGGVDWK RRRHEAPRRH DIPPPHGV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Immediate early (EI) protein that functions as a transcriptional regulator of cellular and viral mRNAs mainly by interacting with several general transcription factors thereby disorganizing the preinitiation complex at certain promoters. May additionally help to regulate levels of histones in virus-infected cells by interacting with host ASF1. By inhibiting host transcriptional program, IE63 plays a major role in the ability of VZV to overcome the innate immune response to the virus.
Subcellular Location
Host cytoplasm. Host nucleus. Virion tegument. Note=During the first stage of infection, IE63 is mostly expressed in the nucleus and also slightly in the cytoplasm, and during latency, IE63 localizes in the cytoplasm quite exclusively.
Protein Families
Herpesviridae ICP22 family
Database Links

KEGG: vg:1487700

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