Recombinant Xenopus laevis Aurora kinase B-A (aurkb-a)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

aurkb-a

Uniprot No.

Alternative Names

aurkb-a; airk2-aAurora kinase B-A; EC 2.7.11.1; Aurora/IPL1-related kinase 2-A; AIRK2-A; XAIRK2-A; Serine/threonine-protein kinase 12-A; Serine/threonine-protein kinase aurora-B-A; xAurora-B

Species

Xenopus laevis (African clawed frog)

Expression Region

1-361

Target Protein Sequence

MSYKENLNPS SYTSKFTTPS SATAAQRVLR KEPYVSTFTT PSDNLLAQRT QLSRITPSAS SSVPGRVAVS TEMPSQNTAL AEMPKRKFTI DDFDIGRPLG KGKFGNVYLA REKQNKFIMA LKVLFKSQLE KEGVEHQLRR EIEIQSHLRH PNILRMYNYF HDRKRIYLML EFAPRGELYK ELQKHGRFDE QRSATFMEEL ADALHYCHER KVIHRDIKPE NLLMGYKGEL KIADFGWSVH APSLRRRTMC GTLDYLPPEM IEGKTHDEKV DLWCAGVLCY EFLVGMPPFD SPSHTETHRR IVNVDLKFPP FLSDGSKDLI SKLLRYHPPQ RLPLKGVMEH PWVKANSRRV LPPVYQSTQS K

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Phosphorylates 'Ser-10' of histone H3 during mitosis.

Gene References into Functions

AurB as an important RNA-dependent spindle assembly factor PMID: 24968351
Data suggest a model in which microtubules act as scaffolds for the enzymatic activity of Aurora-B. PMID: 24498282
ICIS and Aurora B coregulate the microtubule depolymerase Kif2a. PMID: 19327998
results suggest an essential combined function of AurA and AurB in chromosome segregation and anaphase MT dynamics PMID: 22184196
Aurora B pathway is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activated by chromatin via a Ran-independent mechanism. PMID: 17199039
Aurora kinase B controls spindle bipolarity via inhibition of endogenous MCAK. PMID: 22751439

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Subcellular Location

Nucleus. Chromosome. Note=Chromosomal until metaphase but transfers to the midzone microtubule array and the equatorial cortex during anaphase.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily

Database Links

KEGG: xla:398457

UniGene: Xl.6652

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Recombinant Xenopus laevis Aurora kinase B-A (aurkb-a)

Product Details

Customer Reviews and Q&A

Target Background

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