Recombinant Xenopus laevis DDB1- and CUL4-associated factor 12-A (dcaf12-a)

Code CSB-YP731020XBE
MSDS
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Source Yeast
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Code CSB-EP731020XBE
MSDS
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Source E.coli
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Code CSB-EP731020XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP731020XBE
MSDS
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Source Baculovirus
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Code CSB-MP731020XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dcaf12-a
Uniprot No.
Alternative Names
dcaf12-a; wdr40a-aDDB1- and CUL4-associated factor 12-A; WD repeat-containing protein 40A-A
Species
Xenopus laevis (African clawed frog)
Expression Region
1-446
Target Protein Sequence
MTRRSVSRKR RANPGSGPGE QSDWDHSAHK RKRLPPEKKS LVFYLKSREL KPHNDSTYLH LLRGHAACTL PSILSEREFH LGNLNKVFAS QWLNHRQVVC GTKCNTLFVV DVQTGQITRI PILKDREPIN GSHQSCGIHA IEINPSRTLL ATGGDNPNSI AVYRLPTLDP VCVGDGGHND WIFSIAWISD TMAVSGSRDG SMGLWEMTDE VVNKSDFQHG LSRVPVYSHI SHKALKDIPK ESSNPVNCKV RALAFNSNNK ELGAVSLDGF FHLWKAEQTL SKLLSTKLPY CRENVCLAYG LEWSLYAVGS QAHVSFLDPR QPPHCAKSVY CREQGSGIRS VSFYEHIVTV GTGQGALLFY DIRAQRFLED STGNCRNAKK KGDTLKLSTG KGWLNHNEMW LNYFSDIDCC PNAVYTHCYD SSGTKLFVAG GPLPTGLHGN YAGLWS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, leading to their ubiquitination and degradation.
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
WD repeat DCAF12 family
Database Links

KEGG: xla:494761

UniGene: Xl.3334

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