Recombinant Xenopus laevis Neurogenic differentiation factor 1(neurod1)

Code CSB-YP856245XBE
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Source Yeast
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Code CSB-EP856245XBE
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Source E.coli
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Code CSB-EP856245XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856245XBE
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Source Baculovirus
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Code CSB-MP856245XBE
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names neurod1
Uniprot No. Q91616
Alternative Names neurod1; neurod; Neurogenic differentiation factor 1; NeuroD1
Species Xenopus laevis (African clawed frog)
Expression Region 1-352
Target Protein Sequence MTKSYGENGL ILAETPGCRG WVDECLSSQD ENDLEKKEGE LMKEDDEDSL NHHNGEENEE EDEGDEEEED DEDDDEDDDQ KPKRRGPKKK KMTKARVERF KVRRMKANAR ERNRMHGLND ALDSLRKVVP CYSKTQKLSK IETLRLAKNY IWALSEILRS GKSPDLVSFV QTLCKGLSQP TTNLVAGCLQ LNPRTFLPEQ SQDIQSHMQT ASSSFPLQGY PYQSPGLPSP PYGTMDSSHV FHVKPHSYGA ALEPFFDSST VTECTSPSFD GPLSPPLSVN GNFTFKHEHS EYDKNYTFTM HYPAATISQG HGPLFSTGGP RCEIPIDTIM SYDGHSHHER VMSAQLNAIF HD
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Acts as a transcriptional activator. Plays a role as a differentiation factor during neurogenesis. Required for the conversion of cells within the neural plate and ventral ectoderm into differentiated neurons. Promotes the differentiation of late-born retinal amacrine cells, but not early-born retinal ganglion cells. Its function is inhibited at early stages of retinal cells differentiation.
Gene References into Functions
  1. Premature misexpression of NeuroD1 in chick partially recapitulates the amphibian condition by suppressing transit amplification. PMID: 25005474
  2. NeuroD1 gene activity is turned on in new born neurons during post-metamorphic neurogenesis. PMID: 23799108
  3. NeuroD promoter is substantially more sensitive to the phosphorylation status of Ngn2 than the Delta promoter, and that this can be attributed to differences in the ease of promoter activation. PMID: 22491944
  4. This study reveals that the early larval stage in Xenopus (Stage 48) displays patterns of proliferation (NeuroD). PMID: 16025451
  5. Data demonstrate that Neurogenin and NeuroD preferentially recognize neurogenesis-related targets through an enhancer signature of clustered consensus-binding sites and regulate neurogenesis by activating a core set of transcription factors. PMID: 18007592

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed in differentiating neurons of both the central and peripheral nervous systems.
Database Links

KEGG: xla:378541

UniGene: Xl.330

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