Recombinant Zaire ebolavirus Polymerase cofactor VP35 (VP35)

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Code CSB-MP764950ZAT
Size $660
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
VP35
Uniprot No.
Research Area
others
Alternative Names
VP35Polymerase cofactor VP35; Ebola VP35; eVP35
Species
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Source
Mammalian cell
Expression Region
1-340aa
Target Protein Sequence
MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLDSTTSLTEENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
41.4kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The Recombinant Zaire Ebola virus VP35 protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of VP35 gene. This recombinant VP35 protein was expressed in the host. The expression region is 1-340aa of the Zaire Ebola virus VP35. In the production, the expression vector contains N-terminal 10xHis tag and C-terminal Myc tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

VP35, an essential viral RNA-dependent RNA polymerase cofactor, is indispensable for Ebola viral replication and host innate immune escape. VP35 was demonstrated to be phosphorylated at Serine/Threonine by immunoblotting, and the major phosphorylation sites was S187, S205, T206, S208 and S317 as revealed by LC-MS/MS. VP35, which contains both dsRNA-binding and oligomerization domains, is an essential polymerase cofactor and plays immune antagonist roles in host cells by targeting RIG-I-like receptors, PKR, and dsRNA. Previous work indicates VP35 binds synthetic dsRNA molecules in vitro and can inhibit IFN induction mediated by these dsRNAs in cell culture. Several studies clearly demonstrate the correlation between VP35 dsRNA-binding activity and its ability to inhibit type I IFN responses. In addition to acting as an immune antagonist, VP35 also has diverse functions during virus replication. Since virulence is correlated to the ability of the virus to suppress the innate immune response and VP35 is one of the major viral players in suppressing the host innate immune response.

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Target Background

Function
Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1. VP35-NP interaction controls the switch between RNA-bound NP and free NP and thus the switch between genome replication and genome packaging into the nucleocapsid. Prevents establishment of cellular antiviral state, thereby suppressing host DC maturation. Acts by inhibiting host DDX58/RIG-I activation both by shielding dsRNA from detection and by preventing PRKRA binding to DDX58. Blocks virus-induced phosphorylation and activation of interferon regulatory factor 3/IRF3, a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR. Increases PIAS1-mediated SUMOylation of IRF7, thereby repressing interferon transcription. Also acts as a suppressor of RNA silencing by interacting with host DICER1, TARBP2/TRBP and PRKRA/PACT. As a dimer, binds and sequesters dsRNA contributing to the inhibition of interferon production.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Filoviridae polymerase cofactor VP35 family
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