Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
VP35Polymerase cofactor VP35; Ebola VP35; eVP35
Species
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Expression Region
1-340aa
Target Protein Sequence
MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLDSTTSLTEENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Zaire Ebola virus VP35 protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of VP35 gene. This recombinant VP35 protein was expressed in the host. The expression region is 1-340aa of the Zaire Ebola virus VP35. In the production, the expression vector contains N-terminal 10xHis tag and C-terminal Myc tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.
VP35, an essential viral RNA-dependent RNA polymerase cofactor, is indispensable for Ebola viral replication and host innate immune escape. VP35 was demonstrated to be phosphorylated at Serine/Threonine by immunoblotting, and the major phosphorylation sites was S187, S205, T206, S208 and S317 as revealed by LC-MS/MS. VP35, which contains both dsRNA-binding and oligomerization domains, is an essential polymerase cofactor and plays immune antagonist roles in host cells by targeting RIG-I-like receptors, PKR, and dsRNA. Previous work indicates VP35 binds synthetic dsRNA molecules in vitro and can inhibit IFN induction mediated by these dsRNAs in cell culture. Several studies clearly demonstrate the correlation between VP35 dsRNA-binding activity and its ability to inhibit type I IFN responses. In addition to acting as an immune antagonist, VP35 also has diverse functions during virus replication. Since virulence is correlated to the ability of the virus to suppress the innate immune response and VP35 is one of the major viral players in suppressing the host innate immune response.