Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
VP35Polymerase cofactor VP35; Ebola VP35; eVP35
Species
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Expression Region
1-340aa
Target Protein Sequence
MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLDSTTSLTEENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Zaire ebolavirus VP35 contains amino acids 1-340. The calculated molecular weight for this VP35 protein is 42.4 kDa. This VP35 recombinant protein is manufactured in e.coli. Fusion of the N-terminal 10xHis tag and C-terminal Myc tag into the VP35 encoding gene fragment was conducted, allowing for easier detection and purification of the VP35 protein in subsequent stages.
Zaire ebolavirus polymerase cofactor VP35 is a critical component of the viral RNA synthesis machinery during Ebola virus infection. VP35 mainly acts as a polymerase cofactor and a dsRNA-binding protein and is essential for viral replication and immune evasion. Studying VP35 provides insights into viral replication mechanisms, host-virus interactions, and antiviral drug design. In virology, VP35 research contributes to understanding Ebola virus pathogenesis and developing antiviral strategies. In immunology, VP35's role in modulating host immune responses is relevant for vaccine development. Additionally, understanding VP35's immunomodulatory functions offers avenues for developing therapeutic interventions and vaccines against the Ebola virus and related filoviruses.
