Recombinant Zinc finger-interacting factor 1 (zif-1)

Code CSB-YP327331CXY
MSDS
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Source Yeast
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Code CSB-EP327331CXY
MSDS
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Source E.coli
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Code CSB-EP327331CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327331CXY
MSDS
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Source Baculovirus
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Code CSB-MP327331CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
zif-1
Uniprot No.
Alternative Names
zif-1; tag-6; F59B2.6Zinc finger-interacting factor 1; Protein zif-1
Species
Caenorhabditis elegans
Expression Region
1-489
Target Protein Sequence
MSECSASTSQ LSTFCVNEIP WKLVPQTCDE FSEYEFIDEK YRRLSDVESF VWKWSPCGRY GIDLPGGRDV INQQLRDRMA VFDLHTNKWT LYTMDRGAFE IPRVCFLYWA RNDIIGTITL VKDYEAGPDD EIPLKFKQSF FYVSHETQTL VYSGSCKYRT NKTIVASYWY HIFENRRGEP FIDHNNYLWL VFSNGVDSLI LLPFIPTSTC FKAKCVSFDM NDIVSQFTGI RVFLDPFVSS HYVPWVYRND VNFFIRIDSH SFIEEQNRDY GLDPQIDNPE TKPHRGVFQL RVNLKDVIEK GEKDAIHAVN PETIETRFNR GDIELWRKIS DELRQEHQSI AQHGKMVVVQ RWSHFTVDMG LSKKMRTRVA DLDARDYCCS HKPRNSSFCC IDLDSERIKA VDALILGTDV VLPHPSGSVF LFRYKENYGM SYCELPFFQP LSLKLKCIQV LKESVEIDPS YYRIKCCLKM QEELEKETAE NLCTINIQQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable substrate recognition component of a CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ensures germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. This process also requires elc-1, cul-2, rbx-1 and ubc-2.
Gene References into Functions
  1. It was shown that ZIF-1 expression in embryos is regulated by five maternally-supplied RNA-binding proteins. POS-1, MEX-3, and SPN-4 function as repressors of ZIF-1 expression, whereas MEX-5 and MEX-6 antagonize this repression. PMID: 22265679
  2. zif-1 encodes the substrate-binding subunit of the E3 ligase for PIE-1 degradation during germline specification. PMID: 20826530
  3. results show that five CCCH finger proteins, components of the Caenorhabditis elegans germ plasm, are targeted for degradation by the novel CCCH-finger-binding protein ZIF-1 [ZIF-1] PMID: 12894212
Database Links

KEGG: cel:CELE_F59B2.6

STRING: 6239.F59B2.6

UniGene: Cel.38655

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