Recombinant Zygote defective protein 9 (zyg-9), partial

Code CSB-YP520750CXY
MSDS
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Source Yeast
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Code CSB-EP520750CXY
MSDS
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Source E.coli
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Code CSB-EP520750CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP520750CXY
MSDS
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Source Baculovirus
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Code CSB-MP520750CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
zyg-9
Uniprot No.
Alternative Names
zyg-9; F22B5.7; Zygote defective protein 9
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos. Required for default nucleus positioning in oocytes.
Gene References into Functions
  1. Zyg9, the XMAP215/Dis1 homolog in C. elegans exhibits a polymerase activity which is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region. PMID: 21282620
  2. Positioning of the germinal vesicle is dependent on zyg-9. PMID: 12885567
  3. mutual stabilization between TACC and Zyg-9 proteins is a key feature ensuring microtubule assembly in vivo PMID: 12956950
  4. ZYG-9 and TAC-1 physically interact in vivo, and this interaction is important for the efficient localization of the ZYG-9/TAC-1 complex to centrosomes PMID: 12956952
  5. These findings indicate that ZYG-9-TAC-1 and ZYG-8 act in a partially redundant manner to ensure correct microtubule assembly throughout the cell cycle of early C. elegans embryos. PMID: 17666432

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Subcellular Location
Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Note=Cycles between cytoplasm and centrosome during mitosis in a cell cycle- dependent manner. Centrosomal recruitment requires tbg-1.
Protein Families
TOG/XMAP215 family
Database Links

KEGG: cel:CELE_F22B5.7

STRING: 6239.F22B5.7

UniGene: Cel.17001

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