AKT1/2/3 Recombinant Monoclonal Antibody

Code CSB-RA272392A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate
    All lanes: CD44 antibody at 1:1000
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56 kDa
    Observed band size: 56 kDa
  • IHC image of CSB-RA272392A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.
  • Overlay Peak curve showing Jurkat cells stained with CSB-RA272392A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Target Names
Alternative Names
RAC-alpha serine/threonine-protein kinase (EC (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC, RAC-beta serine/threonine-protein kinase (EC (Protein kinase Akt-2) (Protein kinase B beta) (PKB beta) (RAC-PK-beta), AKT2, RAC-gamma serine/threonine-protein kinase (EC (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG
Species Reactivity
A synthesized peptide derived from Human AKT1/2/3 Antibody
Immunogen Species
Homo sapiens (Human)
Rabbit IgG
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The AKT1/2/3 recombinant monoclonal antibody is created using in vitro expression systems, which are established by cloning the DNA sequences of AKT1/2/3 antibodies obtained from immunoreactive rabbits. The immunogen used in this process is a synthesized peptide derived from the human AKT1/2/3 protein. Subsequently, the genes encoding the AKT1/2/3 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are transfected into host cells to facilitate antibody expression. The AKT1/2/3 recombinant monoclonal antibody then undergoes affinity-chromatography purification and is rigorously tested for functionality in ELISA, WB, IHC, and FC applications, confirming its reactivity with the human AKT1/2/3 protein.

AKT1, AKT2, and AKT3 are closely related isoforms of the AKT protein kinase family. While they share structural similarities and some functional overlap, they exhibit distinct tissue-specific expression patterns and play specialized roles in various cellular processes. AKT1 is widely expressed in various tissues and is involved in the regulation of cell growth, survival, and proliferation. AKT2 is predominantly expressed in insulin-responsive tissues such as skeletal muscle, liver, and adipose tissue, and plays a crucial role in glucose homeostasis and insulin signaling. AKT3 is expressed at higher levels in the brain and nervous system, where it contributes to neuronal development and function.

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